The US Embassy in Ukraine's capital Kyiv was temporarily closed on Wednesday, as the mission received information of a "potential significant air attack."
The move comes a day after Ukraine launched US long-range missiles into Russian territory for the first time on Tuesday, using ATACMS missiles against a facility in the Bryansk region close to the border.
Russia warned on Tuesday that it would respond.
A statement released by the Embassy advised employees to shelter in place and "recommends US citizens be prepared to immediately shelter in the event an air alert is announced."
Further details regarding the potential attack have not been disclosed.
Russia had been warning the West for months that if Washington allowed Ukraine to fire US, British and French missiles deep into Russia, Moscow would consider those NATO members to be directly involved in the war in Ukraine.
On Tuesday, Putin lowered the threshold for a nuclear strike in response to a broader range of conventional attacks, with nuclear risks rising amid the highest tensions between Russia and the West in more than half a century.
US President Donald Trump said an angry Israel "violently lashed out" and attacked Iran's major gas field, a significant escalation in the US-Israeli war, but ruled out further such attacks by Israel unless Iran retaliated further.
Israel reopened the Rafah crossing with Egypt on Thursday after nearly three weeks to allow some wounded Palestinians to leave for treatment, after Gaza medics said Israeli strikes had killed four people in the enclave.
Saudi Arabia reserves the right to act militarily against Iran and any trust with Tehran has been shattered, the Saudi foreign minister Prince Faisal bin Fahran said early on Thursday, after Riyadh was targeted by Iranian ballistic missiles.
Kuwait Petroleum Corporation says one of the operational units at the Mina Al Ahmadi refinery was hit on Thursday by a drone, resulting in a "limited" fire.
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