The US Embassy in Ukraine's capital Kyiv was temporarily closed on Wednesday, as the mission received information of a "potential significant air attack."
The move comes a day after Ukraine launched US long-range missiles into Russian territory for the first time on Tuesday, using ATACMS missiles against a facility in the Bryansk region close to the border.
Russia warned on Tuesday that it would respond.
A statement released by the Embassy advised employees to shelter in place and "recommends US citizens be prepared to immediately shelter in the event an air alert is announced."
Further details regarding the potential attack have not been disclosed.
Russia had been warning the West for months that if Washington allowed Ukraine to fire US, British and French missiles deep into Russia, Moscow would consider those NATO members to be directly involved in the war in Ukraine.
On Tuesday, Putin lowered the threshold for a nuclear strike in response to a broader range of conventional attacks, with nuclear risks rising amid the highest tensions between Russia and the West in more than half a century.
Iran said on Thursday it was reviewing Washington's latest position on ending the war after US President Donald Trump suggested he was prepared to wait a few days to "get the right answers" from Tehran but warned of renewed attacks if it did not agree to a deal.
United Nations Secretary-General António Guterres has strongly condemned Israel's decision to establish military facilities in one of the UN's compounds in the Sheikh Jarrah neighbourhood of East Jerusalem.
Israel's treatment of detained Gaza flotilla activists is "abominable", Canadian Prime Minister Mark Carney said Wednesday, following the release of a video showing Israeli police forcing activists to kneel on the ground in rows with their hands tied behind their backs.
Two Chinese tankers laden with oil exited the Strait of Hormuz on Wednesday, shipping data showed, brightening hopes that the US-Israeli conflict with Iran may soon be resolved after positive comments from president Donald Trump and his deputy.
SpaceX aims to reach 10,000 launches annually within five years, but government officials will need to see improved reliability before approving such an expansion, the head of the Federal Aviation Administration said on Wednesday.
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