Lebanon's Hezbollah said it launched a rocket targeting Mossad headquarters near Tel Aviv on Wednesday, which it says was responsible for assassinating its leaders and blowing up pagers and walkie-talkies used by the group.
Warning sirens sounded in Israel's economic capital Tel Aviv as a single surface-to-surface missile was intercepted by air defence systems after it was detected crossing from Lebanon, the Israeli military said.
There were no reports of damage or casualties and the military said there was no change to civil defence instructions for central Israel.
Warning sirens also sounded in other areas of central Israel, including the city of Netanya.
The group used a medium-range Qadr-1 missile, a first for the current conflict. It is also the first time a Hezbollah missile has reached Tel Aviv.
Hezbollah has fired hundreds of missiles and rockets at Israel in recent days as months of conflict across the border with southern Lebanon has intensified sharply.
The Israeli military has been conducting its heaviest air strikes of the war this week, targeting Hezbollah leaders and hitting hundreds of targets deep inside Lebanon that have killed more than 500 people and wounded more than 1,800.
On Tuesday, a strike in Beirut killed senior Hezbollah commander Ibrahim Qubaisi, who headed the group's missile and rocket force.